Fig. 7: Structure-guided design of potent siRNAs as a cleavage agent to restrict SARS-CoV-2 infection.
From: The architecture of the SARS-CoV-2 RNA genome inside virion
a Diagram of strategy against SARS-CoV-2 infection in Vero cells. b The SARS-CoV-2 copies in the supernatant were reduced to background level upon transfection with siRNAs targeting single-stranded regions (si-1 to si-6). Mock, uninfected cells; si-NC, non-targeting siRNA (control). c qPCR showing the abundance of viral RNA in infected Vero cells. Data in (b) and (c) are mean ± s.e.m.; n = 3 biological replicates, two-tailed, unpaired Student’s t-test. Source data are provided as a Source Data file. d Number of single-stranded bases within the siRNA target regions identified in cells and virions. The color intensity denotes the number of siRNAs. The six siRNAs targeting single-stranded regions and three siRNAs targeting duplex regions are labeled as s-1 to s-6 and d-1 to d-3, respectively. R, Pearson correlation coefficient. P-value was determined by two-sided correlation test.
