Fig. 6: Linked-read DNA sequencing maps somatic mutations to germline haplotypes and clonal evolution maps.
From: Co-evolution of tumor and immune cells during progression of multiple myeloma
a Variant allele frequency clustering of subclonal populations from Patient 58408 SMM and Primary samples. b Somatic mutation VAF-based clonality models for Patient 58408. c Variant allele frequency clustering of subclonal populations from Patient 27522 Primary, Relapse-1, and Relapse-2 samples. d Somatic mutation VAF and haplotype-based clonality model for Patient 27522. e Barcode analysis of two NRAS somatic mutations showing both mutations occurred on Haplotype 2 did not co-occur, suggesting an independent subclonal relationship. Each set of linked reads represents a particular pattern of support for the two somatic NRAS mutations. The number of observed barcodes refers to total barcodes demonstrating the same pattern of NRAS somatic mutations.
