Fig. 4: Inhibition of IL-8 induced neutrophil chemotaxis. | Nature Communications

Fig. 4: Inhibition of IL-8 induced neutrophil chemotaxis.

From: Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms

Fig. 4

a Representative flow-cytometry results showing abN48-IgG1 and abN48-2-IgG1 (FITC-conjugated) specifically bind to human primary neutrophils. Blank: cells without hCXCR2 expression; iso-IgG1-FITC: irrelevant human IgG1 antibody conjugated with FITC. (3 independent biological replicates showed the same results) b Inhibition of abN48-2-IgG1 on IL-8 (10 nM) induced neutrophil chemotaxis was determined by a chemotaxis assay. Blank: media without IL-8 or inhibitor; IL-8: 10 nM IL-8; iso: 10 nM irrelevant human IgG1 antibody; MK: 1 μM small molecule CXCR2 inhibitor MK7123. The values of percentage (%) above the blue and green bars represent the percentage of inhibition of neutrophil chemotaxis induced by IL-8 at various concentrations of abN48-2-IgG1 and 1 μM MK7123, respectively. (n = 3 independent cell samples measured in a single experiment, data presented as dots overlapped with mean (column) ± s.d. (error bars). 3 independent experiments with different samples and antibody concentrations showed consistent results).

Back to article page