Fig. 4: AHL15 overexpression reduces heterochromatin condensation during SE induction. | Nature Communications

Fig. 4: AHL15 overexpression reduces heterochromatin condensation during SE induction.

From: An Arabidopsis AT-hook motif nuclear protein mediates somatic embryogenesis and coinciding genome duplication

Fig. 4

a Visualization of DNA compaction using propidium iodide (PI) staining in cotyledon protodermal nuclei of wild-type and p35S:AHL15 IZEs 3 or 7 days after culture. b Quantification of the percentage of dispersed PI-labeled nuclei (a) according to the classification shown in Supplementary Fig. 7. Dots indicate the values of ten biological replicates per plant line with about 200 nuclei analyzed per replicate, bar indicates the mean, and error bars the s.e.m. Different letters indicate statistically significant differences as determined by one-way ANOVA with Tukey’s honest significant difference post-hoc test. The p-values are provided in the source data file. c, d Visualization of DNA compaction in H1.1-GFP (c) or H2B-GFP (d) labeled nuclei in cotyledon protodermal cells of wild-type and p35S:AHL15 IZEs 3 or 7 days after culture. e Visualization of heterochromatin compaction in nuclei of cotyledon protodermal cells stained with PI or immunostained against the heterochromatin marker H3K9me2 in wild-type and p35S:AHL15 IZEs 3 or 7 days after culture. a, c–e Size bar indicates 6 µm and numbers in images indicate the frequency with which similar images from different samples were obtained in two independent experiments.

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