Fig. 1: Cutaneous HSV-1 infection in Nav1.8-DTA mice.
From: Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection
Control DTA and Nav1.8-DTA mice were infected with HSV-OVA-TK− after flank scarification. a C57BL/6 mice were infected with the HSV-OVA or HSV-OVA-TK− viral strains. Viral titres in the skin were analysed 2 days pi, n = 4 mice per group. b Immunofluorescence staining of Nav1.8-TdTomato mouse skin. Whole-mount skin samples were stained with anti-CD45 antibodies (blue); Nav1.8+ cells express TdTomato (red), n = 2 samples. c Representative pictures of the skin lesion progression in control DTA (top line) and Nav1.8-DTA mice (bottom line). d Size of the skin lesions of control DTA and Nav1.8-DTA mice after HSV-OVA-TK− infection (n = 19–20 mice per group) or e treated with PBS only, after flank scarification (n = 5–9 mice per group) (the data obtained for each mouse are shown in Supplementary Table 1). P values were obtained by mixed-effect analysis followed by Sidak’s multiple comparison test. f Viral titres in the skin of control DTA and Nav1.8-DTA mice after HSV-OVA-TK− infection on days 3 pi (n = 13 DTA mice and n = 11 Nav1.8-DTA mice) and 8 pi (n = 5 DTA mice and n = 6 Nav1.8-DTA mice). Data are presented as mean ± SEM.
