Fig. 2: Single-cell analyses of skeletal muscle mesenchymal progenitors in intact muscle.
From: Direct contribution of skeletal muscle mesenchymal progenitors to bone repair
a Transverse sections of TA muscle from Prx1Cre;RosamTmG mice immunostained with NG2, PDGFRβ, PDGFRα, or CD29 antibodies (magenta). DAPI-labeled nuclei in blue, GFP-labeled Prx1-derived cells in green and Tomato labeled non Prx1-derived cells in red. Representative images of three distinct samples. b Experimental design of scRNAseq experiment. Prx1-derived skeletal muscle cells were isolated from hindlimb skeletal muscles and sorted based on GFP-expression prior to scRNAseq. c UMAP projection of color-coded clustering of Prx1-derived cells reveals nine clusters defining four distinct populations (limited by a black dotted line). d Dotplot of indicated gene expression identifying FAP/MP, tenocyte-like cell, pericyte and Spp1/Lgals3 populations. e FeaturePlot of sub-population marker expression (Cd34, Ly6a, and Prrx1 for FAP/MP, Tnmd, Kera, and Scx for tenocyte like cells, Mylk, Des, and Cspg4 for pericytes and Lgals3 and Spp1 for Spp1/Lgals3 cells). Scale bars: low magnification: 30 μm, high magnification: 10 μm.
