Fig. 2: No differences in pancreatic differentiation efficiencies between WT and mutant cell lines. | Nature Communications

Fig. 2: No differences in pancreatic differentiation efficiencies between WT and mutant cell lines.

From: Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells

Fig. 2

a RT-qPCR analysis of HNF1A transcripts during various stages of pancreatic differentiation. n = 3 independent experiments; p = 0.0173. b Flow cytometry analysis of HNF1A protein expression in differentiated endocrine progenitors and β-like cells. c Flow cytometry analysis of GATA4 and PDX1 protein expression in D13 pancreatic progenitors, HNF1A and PDX1 in D20 endocrine progenitors and HNF1A and INS in D35 β-like cells in WT (red) and mutant (blue) cell lines. n = 3 independent experiments. d Gating strategy used for flow cytometry for gating hPSC-derived pancreatic cells across various linages. The same gating strategy was used for all flow cytometry experiments presented in this paper (Figs. 2b, 2c and S2f). e RT-qPCR analysis of INS transcripts in D35 β-like cells. n = 4 independent experiments. D day of differentiation. WT wild-type, P1 patient 1, P2 patient 2. RT-qPCR quantitative reverse transcription polymerase chain reaction. For all statistical analysis: Error bars represent standard error of mean (SEM). Unpaired one-tailed Student’s t-test was performed. Asterisk indicates P-value < 0.05. n.s. non-significant. “See also Fig. S3.” Source data are provided as a Source data file.

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