Fig. 4: PAT5 and PAT9 S-acylate P2K1.
From: S-acylation of P2K1 mediates extracellular ATP-induced immune signaling in Arabidopsis
a Detection of P2K1 S-acylation and their associated residues in the stable P2K1-HA transgenic plants. The S-acylation levels were detected by an acyl-resin capture (acyl-RAC) assay. Palm- palmitoylated protein, Depalm- depalmitoylated protein. HAM, hydroxylamine, for cleavage of the Cys-palmitoyl thioester linkages. Experiments were repeated three times. b PAT5 and PAT9 S-acylate P2K1 in planta. Protoplasts of Arabidopsis wild-type Col-0, Atpat5 and Atpat9 mutants were transfected with plasmids as indicated, followed by acyl-RAC assays. Experiments were repeated three times. c–e Dynamic S-acylation levels of P2K1 in stable transgenic plants upon eATP addition. The P2K1-HA transgenic plant was crossed with pat5, pat9, NP::PAT9-T107/AS109A/pat9, and NP::PAT9-T107D/S109D/pat9 plants and then infiltrated with 200 μM ATP. All experiments were repeated and analyzed three times with similar results.
