Fig. 4: NR treatment restores youthful metabolism in aged HSC.

a Oxygen consumption rate (OCR) in freshly isolated HSPC from aged (n = 3, biological replicates), NR-treated aged (n = 3, biological replicates) and young (n = 4, biological replicates) mice were measured under basal conditions followed by subsequent addition of the ATPase inhibitor oligomycin, the uncoupling reagent FCCP and the inhibitors of the electron transport chain rotenone/antimycin A (R&A), as indicated. b Basal respiration, ATP production and maximal respiration levels were calculated for HSPC (and throughout the manuscript) as indicated in Supplementary Fig. 5a. c Quantification of OCR in freshly isolated HSC and d determination of basal respiration, ATP production and maximal respiration capacity in aged (n = 3, biological replicates) and NR-treated aged mice (n = 4, biological replicates). e Quantification of OCR in freshly isolated MPP and f determination of basal respiration, ATP production and maximal respiration capacity in aged (n = 3, biological replicates) and NR-treated aged mice (n = 4, biological replicates). Data are mean ± s.e.m and statistical analyses were performed using Ordinary one-way ANOVA, p = 0.023 (overall for HSPC basal respiration), p = 0.0001(overall for HSPC ATP production), p = 0.57 (overall for HSPC maximal respiration capacity) with individual groups compared using Tukey’s multiple comparisons test, p-values indicated for b and two-sided Student’s t-test, p-values indicated for d, f. NR nicotinamide riboside, A aged, A + NR NR-treated aged animal. Source data are provided as a Source Data File.