Fig. 6: Metabolic restoration is dependent on continued administration of NR.

a Schematic representation of the experimental setup. b Quantification of oxygen consumption rate (OCR) in freshly isolated HSPC from aged mice (n = 13, biological replicates), NR-treated aged mice (n = 8, biological replicates), aged mice receiving NR prior to 2 months rest (n = 4, biological replicates) and young mice (n = 10, biological replicates), as described in a. OCR was measured under basic conditions followed by subsequent addition of the oligomycin, FCCP and R&A, as indicated. c Basal respiration, ATP production and maximal respiration levels in aged (n = 13, biological replicates), NR-treated aged (n = 8, biological replicates), aged mice receiving NR prior to 2 months rest (n = 4, biological replicates) and young HSPC (n = 10, biological replicates). Data are mean ± s.e.m. and is a pool from three independent experiments. Statistical analyses were performed using ordinary one-way ANOVA, p < 0.0001 (overall for basal respiration), <0.0001 (overall for ATP production), p = 0.0084 (overall for maximal respiration capacity) with individual groups compared using Tukey’s multiple comparisons test, p-values indicated for c. Only statistically significant comparisons of individual groups are indicated. NR nicotinamide riboside, HSPC hematopoietic stem and progenitor cells, Y young, A aged, A + NR NR-treated aged animal. Source data are provided as a Source Data File.