Fig. 1: PHC1 is highly expressed in both human and mouse pluripotent cells.
From: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus
a PRC1 and PRC2 gene expression was examined by RT-qPCR in HFFs, hESCs, and hiPSCs. ACTIN was used as the house keeping gene and expression was normalized to HFFs. n = 4 independent experiments for PRC1 genes; n = 3 independent experiments for PRC2 genes including EZH1, EZH2, EED, and SUZ12. Error bars represent the s.e.m. Two-tailed unpaired t-tests were used (p = 0.0256 for CBX8, p = 0.0201 for PCGF2, p = 0.0498 for PHC1 in HFFs vs. ESCs, p = 0.0104 for PHC1 in HFFs vs. iPSCs, **p = 0.0022 for EZH2, ***p = 0.0003 for EZH2, **p = 0.0034 for SUZ12, *p = 0.0111 for SUZ12). b Expressions of PRC1 and PRC2 genes and key pluripotency factors POU5F1 and NANOG during embryoid body differentiation (D0–D7) of hPSCs were examined by RT-qPCR. n = 3 independent experiments for CBX2, CBX7, and EZH2; n = 4 independent experiments for the other genes. Error bars represent the s.e.m. c WB analysis of PHC1 and NANOG protein expression in hPSCs and HFFs. d Analysis of the published single-cell RNA-seq data of early human embryos (E5–7) showing expression of NANOG and PHC1 in epiblast (Epi), primitive endoderm (PE), and trophectoderm (TE)32. e Co-immunostaining of Phc1 with Nanog and Sox2, or Gata6 and Sox2 in mouse E4.5 embryos. Scale bars, 31 μm. Source data are provided as a Source Data file.
