Fig. 3: Cellular and transcriptomic characterization of ibrutinib-induced lymphocytosis in patient V and clonal evolution in patient B.

a, b The coronal or axial images from PET/CT scan pre- and during ibrutinib treatment. The size of the spleen was measured and labeled. c, d The kinetics of lymphocyte absolute count (Abs) measured during the course of treatment. Samples subjected to scRNA-seq are labeled. e, f Fish plots showing patterns of clonal evolution of tumors from patients B and V. Clonal evolution was inferred using somatic mutations and DNA copy number alterations identified by deep WES. The representative alterations of each clone are labeled. g, h t-SNE plots (left) and SC3 clustering (right) showing the cellular and transcriptomic characterization of the spleen compartment shift of tumor cells during treatment in patient V (left) and therapy induced evolution in patient B (right). i, j The developmental trajectories of tumor cells along pseudotime in a two-dimensional state-space inferred by Monocle2.