Fig. 5: mGluR1 and mGluR2/3-mediated synaptic currents determine the spiking kinetics.
a Sample cell-attached recording (top) instantaneous firing rate (middle) and synaptic current measured with whole-cell voltage clamp (bottom) in a cell with fast response. b Same as in a but for a cell with intermediate speed response. c Same as in a but for a cell with clear biphasic synaptic current (bottom). d Same as in a but for a cell with slow biphasic response. e Same as in a but for a cell with only a pause in firing. f Half-decay times of firing rates vs. half-decay times of currents and linear fit on a log-log plot (Radj2 = 0.91, slope = 0.88, intercept = −0.22, n = 17). g Peak firing rate vs. peak current amplitude and linear fit on a log-log plot (Radj2 = 0.69, slope = 0.71, intercept = −0.65, n = 17). h Pause duration vs. amplitude of the current at stimulation offset, and linear fit with log10 response variable (black, Radj2 = 0.44, slope = 0.01, intercept = −0.42, n = 20). i Heatmap of peak normalized mGluR1-mediated current (n = 10). j Heatmap of peak normalized mGluR2/3-mediated current (n = 7). k Average synaptic response before (black), after (red) mGluR1 antagonist, and after AMPA/NMDA receptor antagonist wash-ins (gray). Each trace is an average of 8 trials. l Average synaptic response before (top, black) and after (top, blue) mGluR2/3 antagonist wash-in, and their difference (bottom, black). Each trace is an average of 8 trials.
