Fig. 6: Altered expression of dopamine receptors resulting from FoxP2 knockdown.
From: Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbird
a A scatterplot illustrating the correlation between normalized expression of Drd1 and Drd2 in FoxP2+ striatal cells that are also Drd1+ or Drd2+. Each point represents a nucleus. Diagonal line indicates the line of equality. Cells above the line have a ratio of Drd2/Drd1 greater than 1. Cells below the line have a ratio of Drd2/Drd1 below 1. b Distribution and boxplots of normalized expression for Drd1, Drd5, and Drd2 in FoxP2+ MSNs in CS-shScr+ and CS-shFoxP2+ birds. Cells are grouped by the expression of dopamine receptors, e.g., D1+ indicates the cells express Drd1 only and no other dopamine receptor, whereas D1+/D5+ indicates the cells express both Drd1 and Drd5. The expression level of Drd1 in cells from CS-shFoxP2+ birds was significantly lower than in CS-shScr+ birds (p < 0.001, Welch’s t-test) in all Drd1+ cells, as indicated by the leftward shift of the distribution of normalized expression in the CS-shScr+ population. The expression of Drd2 in FoxP2+ MSNs did not differ between the two groups, as no shift in the distribution of normalized expression is seen between the populations. The lower and upper bounds of the boxes indicate the 25th and 75th percentiles; the whiskers extend in either direction from the bound to the furthest value within 1.5 times the interquartile range.
