Fig. 3: bAS1/Sad1 and TG1/Sad3 are co-located close to the telomere of the long arm of chromosome 1.
From: Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals
a A chromosome set from a mitotic metaphase spread. Chromatin, blue; bAS1/Sad1, green; nucleolus organiser regions (labelled with pTa71), red; 5S rDNA loci (labelled with pTa794), white. The nucleolus organiser regions (pTa71) are localised on chromosomes 2 and 3 and the 5S rDNA loci (pTa794) on chromosome 398. Chromosomes 6 and 7 are the shortest chromosomes in the genome, submetacentric in contrast to the chromosome carrying bAS1/Sad1 and significantly shorter. A comparison of the lengths of the chromosome carrying bAS1/Sad1 and the two other unidentified chromosomes in the genome—metacentric chromosome 4 and submetacentric chromosome 5—showed that the chromosome carrying bAS1/Sad1 was significantly longer than the other two and is therefore chromosome 1 (t-test p values <0.01 and <0.001, respectively, n = 8 for each chromosome). b Meiotic pachytene cell: chromatin, blue; bAS1/Sad1, red; TG1/Sad3, green; telomeres, magenta. Homologous chromosomes are paired at this stage. In the enlarged views (boxed regions on the right), bAS1/Sad1 (red) is visible as one fluorescent focus per homologous chromosome with an additional faint focus caused by bleed through coming from the telomere label (magenta). Scale bars: 5 µm. c–e FISH localisation of bAS1/Sad1 (red) and TG1/Sad3 (green) in relation to the telomere (magenta) during meiotic pachytene. Sad1 foci appear closer to the telomere in 28% of the pachytene cells analysed (c); TG1/Sad3 foci appear closer to the telomere in 7% of the cells (d); both foci overlap in 65% of the cells (e). Each pachytene FISH experiment involved 4–5 individual slides, each using a different anther. Scale bars: 10 μm. Source data are provided as a Source Data file.
