Fig. 4: The high complexity 12-gene avenacin cluster has assembled de novo in a region of the A. strigosa genome that does not share synteny with other cereals.
From: Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals
a Alignment of the B. distachyon (BDI), rice (OSA), barley (HVU) and wheat (TAE; DD genome shown) showing lack of synteny in the avenacin cluster region. b DNA sequence identity heatmap of the avenacin pathway genes and the other A. strigosa genes in the region shown in Fig. 2a with the most closely related sequences in B. distachyon (BDI), rice (OSA), barley (HVU) and wheat (TAE) (Table S8). c Circos plot showing the locations of these closest matches on the chromosomes of B. distachyon (blue), rice (green), barley (purple) and wheat (DD genome) (brown). d Locations of plantiSMASH-predicted biosynthetic gene clusters (yellow lines) in the A. strigosa genome. Cluster density scores for 100 Mb-sized sliding windows are shown in red. The avenacin cluster is asterisked in the enlarged view of the terminal region of chromosome 1. e Schematic showing the number of genes and gene super-families per cluster in putative triterpene biosynthetic clusters predicted by plantiSMASH in the genomes of A. strigosa S75 (AST), wheat (TAE), Brachypodium distachyon (BDI), Brachypodium stacei (BST), barley (HVU), rice (OSA), maize (ZMA), Sorghum bicolor (SBI), Panicum hallii (PHA), wild emmer wheat (WEW), and Setaria italica (SIT). The avenacin cluster is shown at the top.
