Fig. 6: STAT1-inducible Nampt in the hematopoietic lineage during B16F10 melanoma.
From: A Stat1 bound enhancer promotes Nampt expression and function within tumor associated macrophages
a B16F10-ova melanoma cell line flank tumor size (see “Methods”) growth rate and endpoint mass in WT or Vav-Cre NRE1 fl/fl (NRE1-KO) mice. b scRNAseq cell cluster analysis using UMAP plots for CD45+ cells isolated from B16F10-ova tumors in WT or NRE1 KO mice. Flow cytometric sort purification gating strategy provided in Supplementary Fig. 6, and Source Data file in Supplementary information. c Nampt expression in selected cell types from scRNAseq from WT or NRE1 KO mice. Box-and-whiskers plots show the distribution of gene expression in individual cells, and the red dot denotes average expression across the cells within the sample. Crossbars, boxes, and whiskers represent median, interquartile range, and total range of Nampt expression, respectively. d Pro-inflammatory and metabolic GSEA signatures in indicated cell types from scRNAseq, with positive enrichment of gene sets in wild-type on the left of each plot relative to NRE1 KO on the right. NES normalized enrichment score, padj adjusted p value: two-tailed, corrected for multiple comparisons using Benjamini–Hochberg method. e B16F10-ova flank tumor size growth rate and endpoint mass in WT or LysM-Cre NRE1 fl/fl (NRE1-KO) mice. Except for b–d, data are representative of three independent experiments. Results presented are the average of at least four biological replicates. For a, results presented are the average of N = 9 or 15 biological replicates, for e, N = 10 or 15 replicates, and are representative of at least two independent experiments. p values were determined by a two-tailed unpaired t-test: *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005. Error bars represent SEM.
