Fig. 5: Bi-directional modulation of lipid metabolism.
a–c Pcsk9 activating guides were introduced by AAV8 to male R26SAM/SAM mice after a baseline bleed (n = 5) with bi-weekly bleeds taken thereafter for serum chemistry. Weekly p-values can be found in Supplementary Table 5. a LDL-cholesterol, b total cholesterol, and c HDL-cholesterol levels were plotted as mean +/− SEM per group. d–e Pcsk9 activating guides were introduced by AAV8 to female R26SAM/SAM mice after a baseline bleed (n = 5) with bi-weekly bleeds taken thereafter for serum chemistry. Weekly p-values can be found in Supplementary Table 5. d LDL-cholesterol, e total cholesterol, and f HDL-cholesterol levels were plotted as mean +/− SEM per group. g–i Homozygous female R26SAM study mice were placed on a HFD for six weeks prior to AAV8Ldlr activating viruses were introduced (n = 4). Weekly p-values can be found in Supplementary Table 5. g LDL-cholesterol, h total cholesterol, and HDL-cholesterol levels were plotted as mean +/− SEM per group. (j-l) Homozygous male R26SAM study mice were placed on a HFD for 6 weeks prior to AAV8Ldlr activating viruses being introduced (n = 5). Weekly p-values can be found in Supplementary Table 5. j LDL-cholesterol, k total cholesterol, and l HDL-cholesterol levels were plotted as mean +/− SEM per group. Statistics: Asterisks (*) indicates significance, and the number of asterisks indicates the number of 0s after the decimal point. One-tailed, unpaired Student’s t-test for (a–l).
