Fig. 3: TVM cells have a unique proliferative and metabolic phenotype.
From: Asymmetric cell division shapes naive and virtual memory T-cell immunity during ageing
A Representative flow cytometry plots showing CD122, CXCR3, NKG2D and CD49d expression on CD8+ T cells in the blood of young and old P14 mice. Frequencies of CD44lo and CD44hi CD8+ T cells expressing high CD122, CXCR3 and NKG2D levels and low CD49d levels are depicted in the bar graphs. Pooled data from two independent experiments (young, n = 4: old, n = 5; aged mice: 70, 90 or 100 weeks old). B Histograms depicting proliferation of purified naive or TVM CD8+ T cells from young or aged mice (CTV-labelled, stimulated on α-CD3, α-CD28 and human Fc-ICAM-1 coated wells). After 42 h, the proliferation profile was analyzed by CTV dilution (left panel) or cells were transferred to uncoated wells for further 30 h in medium containing IL-2, IL-7 and IL-15, until their proliferation kinetics was assessed 72 h post stimulation (right panel). Representative data from one out of two experiments (aged mice: 70 or 82 weeks old). C Oxygen consumption rate (OCR) (upper panel) and extracellular acidification rate (ECAR) (lower panel) of activated CD8+ T cells was measured under basal conditions and in response to indicated drugs. Representative data from one out of two experiments, where data points are technical replicates (young naive, n = 10; old naive, n = 5, old TVM, n = 4; aged mice > 100 weeks old in all experiments). Data are depicted as mean ± SEM. Statistical analysis was performed using the unpaired two-tailed Student’s t test. Exact P values are depicted in the figure.
