Fig. 1: T. gondii LIPIN (TgLIPIN) is a phosphatidate phosphatase localized to parasite cytoplasm and endoplasmic reticulum. | Nature Communications

Fig. 1: T. gondii LIPIN (TgLIPIN) is a phosphatidate phosphatase localized to parasite cytoplasm and endoplasmic reticulum.

From: Toxoplasma LIPIN is essential in channeling host lipid fluxes through membrane biogenesis and lipid storage

Fig. 1

a The C-LIP domain of TgLIPIN is evolutionarily conserved among eukaryotic orthologs and harbors the catalytic motif DVDGT known to be central to PA phosphatase activity. b Graphical scheme of the biochemical function of LIPIN in the glycerolipid biosynthesis pathway in eukaryotes. c The complementation of the yeast triple KO mutant (∆dpp1∆lpp1∆pah1), lacking phosphatidic acid phosphatase activity, with the C-LIP domain of TgLIPIN restores of PA and DAG (n = 3, unpaired t test P values where *P = 0.024 DAG triple mutant vs DAG complemented triple mutant, *P = 0.032 DAG triple mutant vs DAG wild-type, **P = 0.0039 PA triple mutant vs PA complemented triple mutant, *P = 0.039 PA triple mutant vs PA wild type). Data are presented as mean values + /− SEM. d IFA of TgLIPIN-HA (endogenous C-terminal tag) with anti-HA, DAPI, and a transiently expressed DER-1-GFP plasmid shows co-localization of TgLIPIN to the ER of the parasite. Scale bar: 2.0 μm.

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