Fig. 4: TgLIPIN regulates critical levels of PA and other major phospholipids while generating DAG that is directed toward neutral lipid storage. | Nature Communications

Fig. 4: TgLIPIN regulates critical levels of PA and other major phospholipids while generating DAG that is directed toward neutral lipid storage.

From: Toxoplasma LIPIN is essential in channeling host lipid fluxes through membrane biogenesis and lipid storage

Fig. 4

a Relative abundance of PA in TgLIPIN-ikD (nmol/parasites number, +ATc/−ATc; %; n = 3, unpaired t test P values where ****P < 0.00001 relative PA abundance ratio nmol/parasites number +ATc/−ATc at 48 h vs +ATc/−ATc = 1). b Fatty acid composition of PA in TgLIPIN-ikD (+/−ATc) in Mol%; n = 3, unpaired t test P values where **P < 0.0021 PA C16:0 −ATc vs +ATc, ***P < 0.0002 PA C18:0 −ATc vs +ATc. c Relative abundance of DAG (+ ATc/−ATc) is reduced by TgLIPIN depletion after 48 h + ATc (n = 3, unpaired t test P values where ****P < 0.00001 relative DAG abundance ratio nmol/parasites number +ATc/−ATc at 24 h vs +ATc/−ATc = 1). d Nile red staining showed the number of lipid droplet within the parasites after 24 h of + /−ATc (scale bar = 5.0 μm) with (e) a representative bar graph (n = 3, unpaired t test P values where **P < 0.0093 number of lipid droplets per parasite +ATc/−ATc at 1% FBS, ***P < 0.0003 at 10% FBS). f Relative abundance of TAGs in TgLIPIN-ikD (+ATc/−ATc) showing that TAG levels were relatively reduced in the TgLIPIN-ikD (48 h + ATc). g Relative abundance of free fatty acids (FFA, +ATc/−ATc in the TgLIPIN-ikD). h Relative abundance of PLs, DAG, FFA and TAG in the TgLIPIN-ikD +ATc to −ATc at 10% FBS (n = 3, unpaired t test P values where **P < 0.004 relative TAG abundance ratio nmol/parasites number +ATc/−ATc at 10% FBS vs +ATc/−ATc = 1). Data are presented as mean values + /− SEM.

Back to article page