Fig. 2: Glucose metabolism parallels mortality.
From: Feeding-induced resistance to acute lethal sepsis is dependent on hepatic BMAL1 and FXR signalling
a Serum glucose (p < 0.0001) 6 h post LPS (20 mg/kg) stimulation on day 5 of TRF. Female in black, male in red. NF-ZT0 Nfemale = 11 Nmale = 7, NF-ZT12 Nfemale = 8 Nmale = 5, DF-ZT0 Nfemale = 11 Nmale = 7, DF-ZT12 Nfemale = 9 Nmale = 7. Box indicates mean. Statistical significance according to two-way ANOVA and Sidak’s multiple comparison (ZT0 vs. ZT12) as indicated. pNF = 0.0002, pDF = 0.0007. Interaction F(1,66) = 34.21, p < 0.0001, ZT F(1,66) = 0.09264, ns, Feeding F(1,66) = 0.03347, ns. b Liver glycogen content on day 5 of TRF measured at ZT0 or ZT12. Female in black, male in red. Nfemale = 3 Nmale = 5. Box indicates mean. Statistical significance according to two-way ANOVA and Sidak’s multiple comparisons (ZT0 vs. ZT12) as indicated. Interaction F(1,28) = 78.25, p < 0.0001, ZT F(1,28) = 0.006643, ns, Feeding F(1,28) = 0.4853, ns. ****p < 0.0001. c Baseline serum levels of β-hydroxybutyrate (BHB) at the indicated ZT on day 5 of day-time (grey) or night-time feeding (black) (Interaction F(6,61) = 10.31, p < 0.0001, ZT F(6,61) = 2.704, p = 0.0215, Feeding F(1,61) = 0.717, p = 0.4004), NZT0 = 10, NZT2 = 3, NZT6 = 3, NDF-ZT12 = 13, NNF-ZT12 = 12, NZT14 = 3, NZT18 = 3, NZT24 = 3. Each dot represents an individual data point and the line represents the mean. Females only. Statistical analysis using two-way ANOVA and Sidak’s multiple comparisons (NF vs. DF). ***p = 0.0003 ****p < 0.0001. d BHB serum levels 6 h post LPS on day 5 of TRF at ZT0 or ZT12 in female mice. DF-ZT0-LPS N = 11, NF-ZT0-LPS N = 12, DF-ZT12-LPS N = 11, NF-ZT12-LPS N = 11, DF-ZT0-PBS N = 6, NF-ZT0-PBS N = 6, DF-ZT12-PBS N = 6, NF-ZT12-PBS N = 6. Mean is indicated by the box. Statistical analysis using two-way ANOVA (LPS and PBS separately) and Sidak’s multiple comparison (ZT0 vs. ZT12) as indicated. LPS: Interaction F(1,40) = 18.29, p < 0.0001, ZT F(1,40) = 2.357, ns, Feeding F(1,40) = 1.184, ns. PBS: Interaction F(1,20) = 0.001053, ns, ZT F(1,20) = 5.015, p = 0.0367, Feeding F(1,22) = 0.9777, ns. ***p = 0.0004. e, f Serum corticosterone on day 5 of TRF at baseline (e) (Interaction F(1,49) = 20.36, p < 0.0001, ZT F(1,49) = 1.159, ns, Feeding F(1,49) =0.05739, ns, *p = 0.0305 ***p = 0.0007, DF-ZT0 N = 14, DF-ZT12 N = 14, NF-ZT0 N = 14, NF-ZT12 N = 11) or 6 h post LPS (20 mg/kg)/PBS control stimulation (f) (LPS and PBS ns, DF-ZT0-LPS N = 10, DF-ZT12-LPS N = 11, NF-ZT0-LPS N = 8, NF-ZT12-LPS N = 10, DF-ZT0-PBS N = 6, DF-ZT12-PBS N = 6, NF-ZT0-PBS N = 4, NF-ZT12-PBS N = 5) in female mice. Box indicates mean. Statistical analysis using two-way ANOVA (LPS and PBS separately) and Sidak’s multiple comparison (ZT0 vs. ZT12) as indicated.
