Fig. 3: The myeloid clock is dispensable for feeding time-dependent mortality.
From: Feeding-induced resistance to acute lethal sepsis is dependent on hepatic BMAL1 and FXR signalling
a, b Liver (a) and spleen (b) gene expression profile of selected clock genes measured by NanoString on day 5 of TRF at indicated times in female mice. Unsupervised gene clustering. c, d Morbidity in night-time (c) or day-time (d) fed myeloid cell specific Bmal1ΔMye (solid) (NF ZT0 vs. 12 p = 0.0032, DF ZT 0 vs. 12 ns, ZT0 NF vs. DF p = 0.0005, ZT12 NF vs. DF ns), or control Bmal1fl/fl (dashed line) mice (NF 0 vs. 12 p = 0.0008, DF 0 vs. 12 p < 0.0001, ZT0 NF vs. DF p < 0.0001, ZT12 NF vs. DF p < 0.0001) following 20 mg/kg LPS i.p. on day 5 of TRF at ZT0 or ZT12 as indicated (Bmal1ΔMye vs. respective Bmal1fl/fl all ns). Statistical analysis according to Mantel–Cox. Experimental numbers: Bmal1ΔMye: DF-ZT0-LPS Nfemale = 7 Nmale = 7, NF-ZT0-LPS Nfemale = 7 Nmale = 7, DF-ZT12-LPS Nfemale = 7 Nmale = 7, NF-ZT12-LPS Nfemale = 6 Nmale = 7, DF-ZT0-PBS Nfemale = 2 Nmale = 4, NF-ZT0-PBS Nfemale = 2 Nmale = 3, DF-ZT12-PBS Nfemale = 1 Nmale = 5, NF-ZT12-PBS Nfemale = 1 Nmale = 4. Bmal1fl/fl: DF-ZT0-LPS Nfemale = 16 Nmale = 16, NF-ZT0-LPS Nfemale = 16 Nmale = 12, DF-ZT12-LPS Nfemale = 15 Nmale = 14, NF-ZT12-LPS Nfemale = 16 Nmale = 14, DF-ZT0-PBS Nfemale = 8 Nmale = 4, NF-ZT0-PBS Nfemale = 8 Nmale = 5, DF-ZT12-PBS Nfemale = 7 Nmale = 5, NF-ZT12-PBS Nfemale = 5 Nmale = 6. e–h Serum cytokines in Bmal1ΔMye and Bmal1fl/fl control mice 2 h post LPS stimulation (20 mg/kg) on day 5 of TRF at ZT0 or ZT12 as indicated. Female in black, male in red. Box indicates mean. Statistical analysis according to two-way ANOVA (within genotype) and Sidak’s multiple comparison (ZT0 vs. ZT12) as indicated. e Bmal1fl/fl: ns. Bmal1ΔMye: Interaction F(1,38) = 4.234, p = 0.0465, ZT F(1,38) = 2.668, ns, Feeding F(1,38) = 1.424, ns. Bmal1fl/fl: NF-ZT0 Nfemale = 6 Nmale = 4, NF-ZT12 Nfemale = 6 Nmale = 6, DF-ZT0 Nfemale = 7 Nmale = 6, DF-ZT12 Nfemale = 7 Nmale = 6. Bmal1ΔMye: NF-ZT0 Nfemale = 6 Nmale = 6, NF-ZT12 Nfemale = 3 Nmale = 7, DF-ZT0 Nfemale = 6 Nmale = 4, DF-ZT12 Nfemale = 4 Nmale = 6. *p = 0.0221. f Bmal1fl/fl: ns, Bmal1ΔMye: Interaction F(1,52) = 4.574, p = 0.0372, ZT F(1,52) = 1.441, ns, Feeding F(1,52) = 0.6317, ns. *p = 0.0435. g ns. h Bmal1fl/fl: Interaction F(1,52) = 8.776, p = 0.0046, ZT F(1,52) = 1.923, ns, Feeding F(1,52) = 5.985, p = 0.0.0178. Bmal1ΔMye: Interaction F(1,52) = 6.532, p = 0.0136, ZT F(1,52) = 0.9279, ns, Feeding F(1,52) = 4.214, p = 0.0.451. **p = 0.0057. f–h Bmal1fl/fl: NF-ZT0 Nfemale = 7 Nmale = 6, NF-ZT12 Nfemale = 8 Nmale = 6, DF-ZT0 Nfemale = 7 Nmale = 8, DF-ZT12 Nfemale = 7 Nmale = 7. Bmal1ΔMye: Nfemale = 7 Nmale = 7.
