Fig. 3: RFC4 promotes NSCLC metastasis and stemness both in vitro and in vivo.
From: An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness
a The effect of overexpressing RFC4 on the abilities of A549 cells to invade through matrigel or to form tumor spheres. Representative images of three independent reproducible experiments are shown. Scale bar: 50 μm. b The effect of silenced RFC4 on the abilities of H1975, LLC, and LC1 cells to invade through matrigel or to form tumor spheres. Representative images of three independent reproducible experiments are shown. Scale bar: 50 μm. c RFC4-overexpressing A549-luci cells were injected via cardiac ventricle into nude mice (n = 5 per group). Representative bioluminescent images of systemic metastasis and ex vivo organ metastases are shown (left panel). H&E histologically confirmed tumor cells in brain and bone tissue (right panel). Two representative cases are shown. Scale bar: 100 μm. d RFC4-slienced H1975-luci cells were injected via cardiac ventricle into nude mice (n = 5 per group). Representative bioluminescent images of systemic metastasis and ex vivo organ metastases are shown (left panel). H&E histologically confirmed tumor cells in brain and bone tissue (right panel). Two representative cases are shown. Scale bar: 100 μm. e Growth curves of tumor xenografts of RFC4-overexpressing A549 cells subcutaneously implanted with different cell numbers and tumor formation frequencies for indicated cell numbers are shown. f C57BL/6N mice (n = 5 per group) were intravenously injected with RFC4-silenced LLC cells. Micro-CT imaging and H&E staining of two representative cases’ lung tissue are shown. Scale bar: 100 μm. g Tumor xenografts of RFC4-silenced LLC cells subcutaneously implanted with different cell numbers and tumor formation frequencies for indicated cell numbers are shown (n = 5 per group). Three representative cases are shown. Scale bar: 2 mm. Data in panel e were presented as mean ± SD derived from three independent experiments. Two-way ANOVA multiple comparison analysis was used for statistical analysis. Source data are provided as a Source Data file.
