Fig. 6: RFC4-induced stabilization of NICD1 promotes NSCLC aggressiveness and resists treatment with γ-secretase inhibitor.
From: An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness
a, b The effect of silencing RFC4 on Notch signaling activities in A549, H1975, LC1, and LLC cells transfected with HA-tagged wild type or stabilized NICD1. Representative images of three independent reproducible experiments are shown. c A549 cells overexpressing stabilized NICD1 or together with RFC4 silencing were intravenously injected into nude mice (n = 5 per group). Representative images of picric acid staining, H&E staining, and the numbers of metastatic foci of indicated lung tissue are shown. Scale bar: 100 μm. d Representative TUNNEL and IHC staining images of CCND1, PCNA, NICD1, and RFC4 in tumor xenografts of the indicated cells (n = 5 per group). e The effect of silencing Notch1 in A549 cells overexpressing RFC4 on the metastatic and tumorigenic abilities. Scale bar: 100 μm. f, g The effect of DAPT treatment in A549 and H1703 cells overexpressing Notch1, wild-type NICD1, or RFC4 on their abilities to form tumor spheres or to invade through matrigel. Scale bar: 50 μm. Data in panel b, c, e–g are presented as mean ± SD derived from three independent experiments. Two-way ANOVA multiple comparison analysis was used for statistical analysis. Source data are provided as a Source Data file.
