Fig. 3: SF Treg cells adapt to the interferon-skewed inflammatory environment. | Nature Communications

Fig. 3: SF Treg cells adapt to the interferon-skewed inflammatory environment.

From: Conserved human effector Treg cell transcriptomic and epigenetic signature in arthritic joint inflammation

Fig. 3

a Unsupervised principal component analysis (PCA) of synovial fluid (SF)-derived Treg cells and non-Treg cells (paired, n = 4) and peripheral blood (PB)-derived Treg cells (n = 3) from healthy children. b Gene ontology terms related to the 1791 genes specifically upregulated in SF (n = 4) compared to PB Treg cells derived from children (active juvenile idiopathic arthritis (JIA, (n = 3)), inactive JIA (n = 4), and healthy children (n = 3)), ranked by enrichment scores. The number of upregulated genes compared to the total number annotated in the gene ontology term are depicted before the terms. c mRNA expression (log2 RPKM) of TBX21, CXCR3, and IL12RB2 in Treg cells derived from PB of healthy adults (adult, n = 4), healthy children (child, n = 3), JIA patients with active (aJIA, n = 3) or inactive (iaJIA, n = 4) disease, SF of JIA patients (JIA SF, n = 4) and non-Treg cells from PB of healthy adults (n = 4) and SF of JIA patients (n = 4) (shown are median + IQR, de = differentially expressed according to the description in Fig. 1d). Adj p-value TBX21 = 0.91, CXCR3 = 0.44, IL12RB2 = 6.83E−10. d Median Fluorescence Intensity (MFI) of T-bet, CXCR3, and IL12Rβ2 in CD3+CD4+CD25+CD127low Treg cells and CD3+CD4+CD25−CD127+ non-Treg cells from paired SFMC and PBMC from 5 JIA patients. e Representative contourplot, for two independent experiments, of T-bet and FOXP3 in CD3+CD4+CD25+FOXP3+ Treg cells and CD3+CD4+CD25int/−FOXP3− non-Treg cells from SFMC. f Percentage of IFNγ and IL-2 positive cells measured in CD3+CD4+CD25+FOXP3+ Treg cells and CD3+CD4+CD25int/−FOXP3− non-Treg cells from SFMC and PMBC (IFNγ: n = 11 PB, n = 12 SF; IL-2: n = 6 PB, n = 7 SF, mean ± SEM, p-values: IFNγ PB p > 0.9999, IFNγ SF p < 0.0001, IL-2 PB p = 0.0051, IL-2 SF p < 0.0001). d, f Data are representative of two independent experiments. Statistical comparisons were performed using two-way ANOVA with Sidak correction for multiple testing. Source data are provided as a Source Data file and deposited under GSE161426.

Back to article page