Fig. 4: Sand fly salivary yellow proteins exacerbate Leishmania infection.
From: A sand fly salivary protein acts as a neutrophil chemoattractant
a–c C57BL6 mice ears were infected with L. major metacyclics (500 parasites) in PBS or with rPduM10 + rPduM35 (0.125 μM of each), P. duboscqi SGH (0.5 pair) or rPduM34 (0.25 μM). a Ear thickness was measured every week. Parasite load assessed by limiting dilution assay at b 1 or c 7 weeks post infection. d, e C57BL/6 mouse ears were infected with L. major metacyclics (500 parasites) in PBS or with 0.5 pair equivalent of P. duboscqi SGH premixed or not with antibodies against PduM10 or PduM35 (1:100 dilution). Parasite load assessed by limiting dilution assay at 1 (d) or 6 weeks (e) post infection. f Anti-Ly6G (clone 1A8) antibody was administered intraperitoneally to C57BL/6 mice 18 h before infection. C57BL6 mouse ears were infected with L. major metacyclics (500 parasites) in PBS or with rPduM10 + rPduM35 (0.125 μM of each). Parasite load assessed by limiting dilution assay 7 weeks post infection. Cumulative results of two independent experiments are presented as a means ± SEM or b–f geometric mean ± geometric SD; a n = 20, 20, 18, 20. a P = 0.031, b P = 0.024, c P = 0.0002. d P = 0.0013. e, f, h, j P < 0.0001. g P = 0.0149. i P = 0.021. k P = 0.0028. b n = 20; **P = 0.0014, ***P = 0.0002, ****P < 0.0001. c n = 18, 18, 20, 20; *P = 0.162, **P = 0.0014, ***P = 0.0005, ****P < 0.0001. d n = 18, 18, 17; **P = 0.0027, ****P < 0.0001. e n = 9, 10, 10; **P = 0.0017, ***P = 0.0001. f n = 20; ****P < 0.0001, calculated by a two-way ANOVA test, b–e one-way ANOVA or f two-tailed Mann–Whitney test.
