Fig. 2: Quantification of how barrier-aligned nucleosome array features depend on barrier type, remodeler type, and nucleosome density. | Nature Communications

Fig. 2: Quantification of how barrier-aligned nucleosome array features depend on barrier type, remodeler type, and nucleosome density.

From: Ruler elements in chromatin remodelers set nucleosome array spacing and phasing

Fig. 2

a Composite plots of same MNase-seq data for INO80 and in vivo as in Fig. 1d but aligned at in vivo Reb1 sites (left), or at BamHI sites (right) of SGD chromatin reconstituted at the indicated nucleosome densities and incubated with INO80 and BamHI. b Scheme defining array features quantified for barrier-aligned composite plots as in a. Array feature values for the indicated combinations of barrier, remodeler, and nucleosome density plotted in different ways allowing comparison between barriers (especially (c)), values (especially (d)), and remodelers (especially (e)). Chd1 refers to the Chd1/FACT complex. Every dot in ce represents one replicate; the lines in c and e and the numbers in d indicate the mean. Replicate dots are often hidden underneath numbered box in d. Composite plots of individual replicates for data shown in a, ce in Supplementary Fig. 2c.

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