Fig. 3: Glutamate dose–response curve in the presence of glycine.

a Representative smFRET traces in the presence of 100 µM glycine (top), 100 µM glycine plus intermediate 300 nM glutamate (middle), and 100 µM glycine plus saturating 1 mM glutamate (bottom). b FRET histograms recorded with increasing glutamate concentration in the presence of 100 µM glycine. Each histogram n = 5 movies, SEM error bars. c Dose–response curve for glutamate in the presence of 100 µM glycine (n = 5 movies, SEM error bars).