Fig. 5: TGFβ converts Nur77 role in regulating ID1 expression. | Nature Communications

Fig. 5: TGFβ converts Nur77 role in regulating ID1 expression.

From: Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis

Fig. 5

a, b, d, f, h HCT116 cells transfected with the indicated expression plasmids or siRNA were treated with TGFβ (10 ng/mL) for 1 h. Protein interactions were examined by co-IP. si-ctr control siRNA, si-Smad3 Smad3 siRNA. c HCT116 cells transfected with the indicated siRNAs were treated with MG132 for 2 h prior to TGFβ (10 ng/mL) treatment for 1 h. ID1 ubiquitylation was examined. si-Nur77 Nur77 siRNA. e, g LS174T cells were pretreated with flavopiridol (0.25 μM) for 1 h (g), and stimulated by TGFβ (10 ng/mL) for 1 h. Protein interactions were examined by co-IP. Data represent at least two independent experiments. Source data are provided as Source Data file.

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