Table 1 The crystal structure information of IiPLR1, AtPrR1, and AtPrR2 proteins in their apo, NADP+ and substrate/product binding forms.
From: Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
apo | NADP+ | NADP+ | NADP+ | NADP+ | ||||
|---|---|---|---|---|---|---|---|---|
(+)-Pinoresinol | (−)-Pinoresinol | (+)-Lariciresinol | (−)-Lariciresinol | (+)-Secoisolariciresinol | (−)-Secoisolariciresinol | |||
IiPLR1 | IiPLR_apo 2.7 Å | IiPLR1_NAP 2.4 Å | IiPLR1_NAP_ + PIN 2.3 Å | IiPLR1_NAP_-PIN 2.1 Å | – | IiPLR1_NAP_-LAR 2.2 Å | IiPLR1_NAP_ + SEC 2.3 Å | IiPLR1_NAP_-SEC 2.6 Å |
AtPrR1 | AtPrR1_apo 2.8 Å | AtPrR1_NAP 2.0 Å | AtPrR1_NAP_ + PIN 2.0 Å | AtPrR1_NAP_-PIN 2.5 Å | AtPrR1_NAP_ + LAR 1.8 Å | AtPrR1_NAP_-LAR 2.5 Å | – | AtPrR1_NAP_-SEC 2.0 Å |
AtPrR2 | AtPrR2_apo 2.0 Å | – | AtPrR2_NAP_ + PIN 1.6 Å | – | – | – | – | – |
