Table 2 Kinetic properties of IiPLR1_V46A.

From: Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases

Substrate

Km (μM)

Vmax (μM/min)

kcat (min−1)

kcat/Km (μM−1 min−1)

(±)-pinoresinol

29.4 ± 1.62

3.22 ± 0.68

114.7 ± 24.1

3.88 ± 0.65

(±)-lariciresinol

26.5 ± 0.60

0.023 ± 0.0013

0.81 ± 0.045

0.031 ± 0.002

  1. Data are expressed as mean ± s.d. with three independent experiments. Source data are provided as a Source Data file.
Back to article page