Fig. 1: Phosphoproteome profiling reveals TOT3/MAP4K4 as a potential regulator of thermomorphogenesis in Arabidopsis.
From: The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis
a Schematic overview of the experimental setup. b Representative seedlings subjected to the experimental setup, after 3 days of exposure to 27 °C. Scale bar, 5 mm. c Venn diagram comparing datasets to define TARGETs OF TEMPERATURE (TOTs), and specifically TOT3. n.d., not determined (plasma membrane localization only predicted for candidates from A. thaliana experiment). Kinases based on ref. 73. d, e Merged red and green signals of localization of GFP:TOT3 (green) in the etiolated hypocotyl of tot3-2 seedlings expressing pTOT3::GFP:TOT3 seedlings grown at 28 °C and stained with the endocytic tracer dye FM4-64 (red) (d). Inset in (d) shows GFP:TOT3 signal alone. Quantitation of relative fluorescence intensity at 493-532 nm (GFP) and 596-645 (FM4-64) along dotted white line (e). Scale bar, 20 µm (d).
