Fig. 6: DA neuron networks with 16p11.2 deletion have increased bursting phenotypes.

a Quantification of the numbers of bursts/min over all sensors. 16pdel neurons have higher number of bursts compared to control or 16pdup neurons, adjusted p values for control vs. 16pdel: p = 0.0016 d18, p < 0.0001 d20 and for 16pdel vs.16pdup: p = 0.0029 d18, p < 0.0001 d20. b Quantification of the number of spikes/burst. Increased spike number/burst was detected in the 16pdel neurons compared to control neurons after 20 days of plating on HD-MEA, adjusted p values for control vs. 16pdel: p = 0.031 d20. c Quantification of the burst duration (s). No significant differences were detected in the burst durations between control, 16pdup, or 16pdel neurons. d Quantification of the time between bursts (s). Shorter time between bursts were detected in the 16pdel neurons compared to control or 16pdup neurons, adjusted p values for control vs. 16pdel: p = 0.0237 d18, p = 0.0049 d20, and for 16pdel vs. 16pdup: p = 0.0471 d20. e Representative raster plots of 200 selected sensors with highest burst rate/chip from the control, 16pdup, and 16pdel groups. f Representative raster plots of 200 selected sensors with most spikes per burst/chip from the control, 16pdup, and 16pdel groups. Datasets were calculated by making interpolation of each data point recorded per chip per day, and averaged data from each group of chips together based on their genotype: control (n = 6), 16pdup (n = 7), and 16pdel (n = 4). Data are presented as mean ± SEM (a–d). Two-way ANOVA followed by Tukey’s multiple comparisons test, and adjusted p values, presented p value *p < 0.05, **p < 0.01, and ****p < 0.0001. Source data are provided as a Source Data file.