Fig. 3: PSD-95 nanocluster organization is modified during NMDA- but not ATP-dependent LTD. | Nature Communications

Fig. 3: PSD-95 nanocluster organization is modified during NMDA- but not ATP-dependent LTD.

From: NMDAR-dependent long-term depression is associated with increased short term plasticity through autophagy mediated loss of PSD-95

Fig. 3

A Example of endogenous PSD-95 organization along a dendritic shaft observe with epifluorescence (top left) or obtained with dSTORM (top right), represented with SR-Tesseler software, Scale bars = 10 µm. Middle panels shows a PSD-95 clusters that have an enrichment factor above the average density factor (density color coded from magenta to yellow). Down panels shows PSD-95 nanoclusters within PSD-95 cluster in the middle panels, which corresponds to a PSD-95 structure with a higher density factor than the average PSD-95 cluster’s density. Scale bars for middle and bottom images (PSD-95 clusters and nanoclusters) = 500 nm (B and C) Average number of PSD-95 molecules per cluster (B) and per nanoclusters (C) in basal state, 10 and 30 min after NMDA treatment (mean ± SEM, n = 17, 19 and 18 respectively, one-way ANOVA p = 0.0059 and Dunnett’s post-test found significant between t0 and t30 conditions, p = 0.0033 but not between t0 and t10 conditions, p = 0.0517 for clusters; one-way ANOVA p = 0.0189 and Dunnett’s post-test found significant between t0 and t10 and between t0 and t30 conditions, p = 0.0348 and p = 0.0194 respectively, for nanoclusters). D and E Average number of PSD-95 molecules per cluster (B) and per nanoclusters (C) in basal state, 10 and 30 min after ATP treatment (mean ± SEM, n = 18, 19 and 16 respectively, one-way ANOVA p = 0.7616 and p = 0.5269 for clusters and nanoclusters respectively).

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