Fig. 6: Effect of AENTH tetramer and mutants on GUVs by fluorescence microscopy. | Nature Communications

Fig. 6: Effect of AENTH tetramer and mutants on GUVs by fluorescence microscopy.

From: Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis

Fig. 6

a, b ANTH-GFP and ENTH-GFP do not bind GUVs without PIP2. c, d ANTH-GFP or ENTH-GFP mixed with ENTH or ANTH, respectively, caused membrane deformation as seen by the numerous membrane protrusions around GUVs. e, f ANTH-GFP or ENTH-GFP did not cause a remodeling effect on the GUV membrane. g–n ANTH and ENTH mutants mixed with ENTH-GFP and ANTH-GFP, respectively, showed different membrane deformation capabilities. Each experiment was repeated independently 3 times with similar results. All scale bars are 5 µm.

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