Table 1 Experimental and theoretical masses for ANTH, ENTH, PIP₂, and adducts/complexes observed by native MS.

Species	Ligand or adduct	Theoretical mass (Da)	Experimental mass (Da)	Main charge state	FWHM^a
–	diC8-PI(4,5)P₂	747.5	–	–	–
ENTH	–	18847.9	18847.8	8+^b	1.2
	+Na	18870.9	18871.4	8+^b	1.1
	+PIP₂	19594.4	19593.2	8+^b	1.1
ANTH	–	33210.0	33209.6	11+	1.2
	+Na	33233.0	33230.3	11+	1.3
	ANTH + PIP₂	33956.5	33959.4	11+	1.3
ANTH₈:ENTH₈ (wildtype)	+22 PIP₂	433181	432887	41+	9.7
	+23 PIP₂	433892	433633	41+	10.5
	+24 PIP₂	434627	434380	41+	9.2
ANTH₆:ENTH₆ (ANTH R3A-I4-D37R-H38A)	+17 PIP₂	324125	324382	36+	8.5
	+18 PIP₂	324872	325141	36+	10.7
	+19 PIP₂	325618	325902	36+	12.2

^aFWHM (full width at half maximum) is given for the predominant charge state peak to indicate the experimental error.
^bSensitivity for the Orbitrap UHMR instrument used here is reduced at low m/z (< 2500), so higher charge states of free ENTH may be attenuated.

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