Table 2 Cryo-EM data collection, refinement, and validation statistics of the AENTH 16-mer assembly.
Data collection and processing | |
|---|---|
Magnification | ×75,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 75.2 |
Defocus range (μm) | −1 to −3 |
Pixel size (Ã…) | 1.065 |
Symmetry imposed | D2 |
Initial particle images (no.) | 195,536 |
Final particle images (no.) | 79,414 |
Map resolution (Ã…) | 3.9 |
FSC threshold | 0.143 |
Map resolution range (Ã…) | 3.7 to 5.7 |
Refinement | |
Initial model used | 5onf, 5oo7 |
Model resolution (Ã…) | 3.9 |
FSC threshold | 0.5 |
Model resolution range (Å) | – |
Map sharpening B factor (Å2) | −200 |
Model composition | |
Non-hydrogen atoms | 26,192 |
Protein residues | 3152 |
Ligands | 20 |
B factors (Ã…2) | |
Protein | 70 |
Ligand | – |
r.m.s. deviations | |
Bond lengths (Ã…) | 0.012 |
Bond angles (°) | 2.037 |
Validation | |
MolProbity score | 1.91 |
Clashscore | 2.60 |
Poor rotamers (%) | 3.78 |
Ramachandran plot | |
Favored (%) | 93.56 |
Allowed (%) | 5.80 |
Disallowed (%) | 0.64 |
