Fig. 3: Proinflammatory cytokine response profiles in Ca-DelMut- and wild-type-virus-infected hamsters. | Nature Communications

Fig. 3: Proinflammatory cytokine response profiles in Ca-DelMut- and wild-type-virus-infected hamsters.

From: Characterization of an attenuated SARS-CoV-2 variant with a deletion at the S1/S2 junction of the spike protein

Fig. 3

Hamsters were infected intranasally with 1 × 103 pfu of either Ca-DelMut (n = 9) or WT HK-13 virus (n = 9). At days 2 and 4, RNA was extracted from lung tissues of infected hamsters (n = 3) and cDNA synthesized using oligo dT primers. Expression of different proinflammatory cytokines was examined by qPCR; relative mRNA levels (relative units) are normalized to an internal reference gene (hamster γ-actin), and the comparative Ct (2-ΔΔCt) method utilized to calculate the cytokine expression profile. Statistical comparisons between means were performed by one-way ANOVA with Tukey post-test: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, NS: not significant.

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