Fig. 4: Structural and histological brain analysis of Fus+/+ and FusΔNLS/+ mice.
a Representation of the workflow used to determine volumes of corresponding brain structures from MRI slices per each mouse by the custom-made Fiji macro plugin (upper row). b Representative MRI slice images of Fus+/+ (upper row) and FusΔNLS/+ (lower row) male mice. c–h Bar graph showing intracranial volume (ICV) (c), normalized volume of the brain parenchyma (d), of lateral ventricles (e), cortex (f), medial septum (g), and cortical subplate (h) in FusΔNLS/+ vs Fus+/+ mice. For panels c–h, N = 5 for Fus+/+ and N = 5 for FusΔNLS/+ mice. Data are presented as mean ± SEM. Two-tailed Unpaired Student’s t-test, c: p = 0.4838; d: p = 0.0249; e: p = 0.0249; f: 0.9489; g: p = 0.0151; h: p = 0.0051. i Representative image of NeuN immunohistochemistry at 22 months of age in Fus+/+ (N = 3 mice) or Fus∆NLS/+ (N = 5) male mice in the anterior region of the M1/M2 cerebral cortex. Scale bar: 100 µm. j Distribution of NeuN+ neurons in Fus+/+ (black) or Fus∆NLS/+ (orange) male mice, in anterior and posterior regions of the M1/M2 cerebral cortex. N = 3 for Fus+/+ and N = 5 for FusΔNLS/+ mice. Source data are provided as a Source Data file.
