Fig. 5: mRNA coexpression network analysis pinpoints defects in inhibitory and excitatory synapses in FusΔNLS/+ mice. | Nature Communications

Fig. 5: mRNA coexpression network analysis pinpoints defects in inhibitory and excitatory synapses in FusΔNLS/+ mice.

From: Cytoplasmic FUS triggers early behavioral alterations linked to cortical neuronal hyperactivity and inhibitory synaptic defects

Fig. 5

a Signed association (Pearson correlation) of the mRNA MEs with transgenic condition. Modules with positive values indicate increased expression in transgenic mice; modules with negative values indicate decreased expression in transgenic mice. The red dotted lines indicate Bonferroni-corrected P < 0.05 for multiple comparisons (n = 12 modules, n = 16 mice per group). b Cell-type enrichment of modules (average n = 200 genes) using mouse genes in mRNA modules (Fisher’s two-tailed exact test, ***FDR = 2 × 10−5). c Coexpression network plot of the synaptic (turquoise) module. The top 12 hub genes are indicated by name. d Gene ontology term enrichment of the synaptic module using 1791 synaptic module genes. e Trajectory of the synaptic module in the cortex of FusΔNLS/+ mice across time. Boxplot show median and quartile distributions, the upper and lower lines representing the 75th and 25th percentiles, respectively. Two-way ANOVA, F(1,24) = 14.55, p = 0.0008; n = 4–6 mice per group. f Coexpression network plot of the splicing/translation module. The top 12 hub genes are indicated by gene name. g GO term enrichment of the splicing/translation module using 1112 splicing/translation module genes. h Trajectory of the splicing/translation module in the cortex of FusΔNLS/+mice across time. Boxplot show median and quartile distributions, the upper and lower lines representing the 75th and 25th percentiles, respectively. Two-way ANOVA, F(1,24) = 11.92, p = 0.002; n = 4–6 mice per group. The center line represents the median.

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