Fig. 4: Age-dependent alterations in the synaptic mRNA profile of FusΔNLS/+ mouse cortex.
From: Synaptic FUS accumulation triggers early misregulation of synaptic RNAs in a mouse model of ALS
a Outline of the RNA-seq experiment. b Heatmap from the set of increased and decreased mRNAs in synaptoneurosomes of FusΔNLS/+ at 6-months compared to Fus+/+. mRNAs are on the rows and the different samples on the columns. The color scale indicates the log2FC between the CPM of each sample and mean CPM of the corresponding Fus+/+ samples at each time point [sample logCPM – mean (logCPM of Fus+/+ samples)]. c Volcano plots showing the log2 fold change of each mRNA and the corresponding minus log10 (FDR) of the differential gene expression analysis comparing total cortex and synaptoneurosomes from FusΔNLS/+ to Fus+/+ at 1 month (left panel) and 6 months of age (right panel). The horizontal line marks the significance threshold of 0.05. Significantly decreased mRNAs are highlighted in green, increased mRNAs in purple and all FUS targets identified in the CLIP-seq data in blue. d Euler diagram of the sets of significantly increased and decreased mRNAs (synaptoneurosomes of FusΔNLS/+ vs. Fus+/+ at 6 months of age) and the synaptoneurosomes FUS targets identified by CLIP-seq. The p-values indicated the enrichment of FUS targets among the increased/decreased mRNAs (one-sided Fisher’s exact test).
