Fig. 3: Pleural macrophages do not invade deep into the lung parenchyma after bleomycin treatment or CI. | Nature Communications

Fig. 3: Pleural macrophages do not invade deep into the lung parenchyma after bleomycin treatment or CI.

From: Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury

Fig. 3

a Schematic figure showing experimental strategies. CI cryoinjury. b Whole-mount bright-field and fluorescent images of lungs after different injuries. Dotted line, CI region. Scale bars, 1 mm. c Sirius red staining of lung tissue sections after bleomycin or cryoinjury in lungs. Boxed regions are magnified. Scale bars, 100 µm. d Immunostaining for tdTomato, GATA6, and F4/80 on injured regions of lungs. m.l., mesothelial layer. Boxed regions are magnified. Scale bars, 100 µm. e, f FACS and quantification analysis of the percentage of macrophages expressing tdTomato from injury regions of the lung. Data are the mean ± SD; n = 5 mice per group. Each image is representative of five individual biological samples (b–d). Source data are provided as a Source Data file.

Back to article page