Fig. 4: Genetic ablation of cavity macrophages did not affect liver repair after injury.

a Schematic figure showing the experimental design. DT diphtheria toxin. b Flow cytometric and quantification analysis of the percentage of CD11b⁺F4/80⁺ macrophages expressing tdTomato from peritoneal cavity after PBS treatment (no DT). Data are the mean ± SD; n = 5 mice per group; ns nonsignificant. c Immunostaining for tdTomato and F4/80 on dissociated cells from the peritoneal or pleural cavity after DT treatment of the G6Mø-CreER;R26-tdTomato/iDTR mice. Quantification of the percentage of F4/80⁺ macrophages expressing tdTomato. Data are the mean ± SD; n = 5 mice per group; ****P < 0.0001. Each image is representative of five individual biological samples. d Flow cytometric and quantification analysis of the percentage of CD11b⁺F4/80⁺ macrophages expressing tdTomato in the pleural cavity, and peritoneal cavity, respectively. Data are the mean ± SD; n = 5 mice per group; ****P < 0.0001. e Schematic figure showing the experimental design. HI heat injury. f Whole-mount bright-field and fluorescence images of livers at 7 days after HI. Boxed regions are magnified; circles, heat injury site. Each image is representative of eight individual biological samples. g Quantification of injury size from 4 h to 7 days after heart injury. Data are the mean ± SD; n = 8 mice per group; ns nonsignificant. h Schematic figure showing the experimental design. i Whole-mount bright-field and fluorescence images of livers at 5 days after CCl₄ treatment. Each image is representative of five individual biological samples. j, Quantification of body weight at different days after CCl₄ treatment. Data are the mean ± SD; n = 5 mice per group; ns, non-significant. Scale bars: yellow, 1 mm; white, 100 µm. P value was calculated by unpaired two-sided Student’s t test (b–d, g) or two-way ANOVA coupled with multiple comparisons (j). Source data are provided as a Source Data file.