Fig. 5: Effects of electrostatics on the effective pore size of P22 PC VLPs.
From: Molecular exclusion limits for diffusion across a porous capsid
All graphs (a–d) are portrayed as the ratio of extracted kcat values for free AhdD-SP enzymes and those encapsulated inside P22 particles versus diameter of NADH conjugates with sigmoidal fits. The data acquired using NADH-Neu are depicted in black, while with NADH-Neg in red. a Effective pore size of 3 nm using neutral dendrimers in pH 7, 50 mM sodium phosphate, 100 mM sodium chloride. b Effective pore size of 4.3 nm using negative dendrimers in pH 7, 50 mM sodium phosphate, 100 mM sodium chloride. c Effective pore size of 3.2 nm using neutral dendrimers in pH 7, 50 mM sodium phosphate, 400 mM sodium chloride. d Effective pore size of 3.0 nm using negative dendrimers in pH 7, 50 mM sodium phosphate, 400 mM sodium chloride. The limits of the y-axis in a–d are not uniform in order to more accurately compare the activity differences between the P22-AdhD and the free enzyme with respect to the size of the NADH conjugates. e Scheme of reaction used in order to neutralize the negative charges on the dendrimers. Error bars = standard deviation (n = 3).
