Fig. 4: High-speed imaging of E. coli biofuel production strains. | Nature Communications

Fig. 4: High-speed imaging of E. coli biofuel production strains.

From: Microsecond fingerprint stimulated Raman spectroscopic imaging by ultrafast tuning and spatial-spectral learning

Fig. 4

a Fingerprint SRS spectra of bovine serum albumin (BSA), pure limonene, and pinene samples. Int., intensity. a.u., arbitrary unit. b–d Fingerprint spectroscopic SRS imaging of E. coli by single raw acquisition, spatial-spectral residual net (SS-ResNet) recovery from raw data, and 100 averaging ground truth (GT). e Quantitative analysis of network recovery quality for the E. coli dataset. Box plots (n = 13) show the NRMSE and SSIM for raw vs. GT and network vs. GT. The boxes show interquartile range (IQR), the red line indicates medians, the black lines represent whiskers which extend to 1.5 times of the IQR and the red data points are the outliers exceeding the whiskers. NRMSE normalized root mean square error, SSIM structural similarity index. f–h Network-recovered image at 1650 cm−1 and chemical mappings of protein, limonene, and pinene for wild type, limonene production, and pinene-production strains. L-production, Limonene production. P-production, Pinene production. Scale bars, 10 µm.

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