Fig. 2: Ecdysone induces a conserved dilp8 expression peak in the cuticle epidermis during pupariation. | Nature Communications

Fig. 2: Ecdysone induces a conserved dilp8 expression peak in the cuticle epidermis during pupariation.

From: The steroid-hormone ecdysone coordinates parallel pupariation neuromotor and morphogenetic subprograms via epidermis-to-neuron Dilp8-Lgr3 signal induction

Fig. 2

a dilp8 transcription peaks at pupariation. Shown are dot plots of qRT-PCR estimations of dilp8 mRNA levels. b dilp8 transcripts are enriched in the white-prepupa (WPP) carcass (integument and body wall muscles). Shown are dot plots of qRT-PCR estimations of dilp8 mRNA levels. c dilp8 and d pale mRNA levels in carcasses treated with 20HE or etOH in vitro. Shown are dot plots of qRT-PCR estimations of mRNA levels of each gene. e Representative photos of puparia from the depicted genotypes. f Knockdown of EcR in epidermal cells with A58 > and Eip71CD > , but not in fat body with ppl > , increases puparium aspect ratio (AR). Shown are dot plots of puparium AR. g Knockdown of EcR in epidermal cells with A58 > and Eip71CD > , but not in fat body with ppl > , reduces dilp8 mRNA expression at the WPP T0 stage. Shown are dot plots of qRT-PCR estimations of dilp8 mRNA levels. h Knockdown of EcR in epidermal cells with A58 > , but not in fat body with ppl > , supresses 20HE-dependent dilp8 transcription in isolated carcasses. Shown are dot plots of qRT-PCR estimations of dilp8 mRNA levels. i C. capitata ilp8 (cilp8) is transiently strongly expressed at WPP T0. Shown are dot plots of qRT-PCR estimations of cilp8 mRNA levels. j In situ hybridization with cilp8 antisense probes stains epidermal cells on C. capitata WPP T0 carcasses. Representative image of at least 3 animals per condition. Statistics (full details in Supplementary Table 2): a, b, c, d, g–i Dots: biological repeats f Dots: one animal. Horizontal bar, median. Error bars: 25-75% percentiles. a, b, f, h, i Same blue letter, P > 0.05. a, b, h, i Student-Newman-Keuls test. f Dunn’s test. g Holm-Sidak test. c, d Student’s t-test. *P < 0.05. (N) Number of animals (orange). Scale bar, 50 µm.

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