Fig. 6: Pupariation progression by coupling morphogenetic and neuromotor subprograms.
a dilp8-mutant puparium aspect ratio (AR) fluctuations are briefer than muscle calcium (mhc»GCaMP) fluctuations during pupariation. Shown are dot plots of PMP duration in dilp8 mutants (−/−) and controls (+/−) according to variation in puparium AR (AR-var) or mhc»GCaMP (GCaMP-var). dilp8(−/−) is dilp8ag52/ag54. dilp8(+/−) is dilp8ag52/ag54. b Post-midthird instar transition-expression of tub > dilp8 delays tanning. Shown are dot plots of the time from GSB to tanning. Red dots, animals performing two GSBs. c Cuticle sclerotization and tanning pathway. mDopa, ɑ-methyldopa. d Photos of puparia. Effects of ɑ-methyldopa. e Quantification of d for dilp8 and f Lgr3 mutants and controls. Shown are dot plots of puparium AR. g ɑ-methyldopa treatment does not rescue GSB of dilp8 or Lgr3 mutants. Shown is the percentage of animals of the depicted genotypes that perform GSB. h ɑ-methyldopa treatment does not rescue the average duration of pre-GSB contractions of dilp8 mutants. Shown are dot plots of the average pre-GSB contraction duration. i Model for the Dilp8-Lgr3-dependent modulation of pre-GSB. j dilp8 mRNA levels increase 5 min after GSB. Shown are qRT-PCR estimations of dilp8 mRNA levels in WT animals. Statistics (full details in Supplementary Table 2): a, b, e, f, j Dots: one animal. h Dots: average per animal. a, e, f, h Horizontal bar, median. Error bars, 25-75%. a, e, h Student–Neuwan–Keuls test. f Dunn’s test. b, j Mann–Whitney Rank sum test. g Binomial tests with Bonferroni correction. a, e, f-h Same blue letters, P > 0.05. *P < 0.05. (N) Number of animals (orange).
