Fig. 3: Retention of TERT intron 11 is robust across cell lines.
From: Nuclear compartmentalization of TERT mRNA and TUG1 lncRNA is driven by intron retention
a UCSC Genome Browser showing RNA-seq coverage across TERT locus (hg38) from multiple cell lines. Scale ln(x + 1). b Maximum intensity projections of representative images of TERT exon/intron smRNA FISH across different cell lines. Exon, gray; introns 2, 11, 14, magenta; nucleus, blue, outlined with a dashed line. Scale bar, 5 μm. Middle: quantification of total and unspliced transcripts for each intron in the nucleus (N) and cytoplasm (C), solid line represents the mean. Right: correlation between nuclear intron and nuclear TERT count; intron 2, black; intron 11, magenta; intron 14, gray. N = 50 cells, at least two independent RNA FISH stainings. c Left: nuclear PIR for each intron across cell lines. Midline line, median; lower and upper box limits, 25th and 75th percentiles; whiskers, 1.5 times interquartile range from the 25th and 75th percentiles. Right: total PIR of each intron and percentage of nuclear enrichment of TERT across cell lines. N = 50 cells, at least two independent RNA FISH stainings.
