Fig. 6: Splicing of TERT intron 11 occurs upon mitosis.
From: Nuclear compartmentalization of TERT mRNA and TUG1 lncRNA is driven by intron retention
a Maximum intensity projections of TERT exon (gray) and intron 11 (magenta) smRNA FISH. Representative images of late prophase, metaphase, anaphase, and telophase are shown. DAPI shown in blue. Scale bar, 5 μm. Three independent experiments. b Quantification of unspliced TERT, spliced (ΔI11) TERT, and free intron 11 in interphase cells and during mitosis. ***P ≤ 0.001, evaluated by unpaired two-tailed t-test (equal variances) versus interphase; n = 20 cells from three independent RNA FISH stainings. c Maximum intensity projections of TUG1 exon (gray) and intron 1 (magenta) or intron 2 (magenta) smRNA FISH. Representative images of metaphases are shown. DAPI shown in blue. Scale bar, 5 μm. Two independent experiments. d, Quantification of unspliced TUG1, spliced (ΔI1 or ΔI2) TUG1, and free intron 1 or 2 in interphase cells and during mitosis. n.s. = not significant, ***P ≤ 0.001, as evaluated by unpaired two-tailed t-test (equal variances) versus interphase; for intron 1 n (interphase) = 30 cells, n (mitosis) = 27 cells; for intron 2 n (interphase) = 29 cells, n (mitosis) = 23 cells; two independent RNA FISH stainings. In b, d midline line, median; lower and upper box limits, 25th and 75th percentiles; whiskers, 1.5 times interquartile range from the 25th and 75th percentiles.
